Coding

Part:BBa_K4488005

Designed by: Sylvie Nolf   Group: iGEM22_Sydney_Australia   (2022-09-30)


Anti-GFP Nanobody/sdAb

Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102

Usage Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488027

Modelling of binding of Nanobody H (3OGO-H) to sfGFP made using python package LightDock

References: Twair, A., Al-Okla, S., Zarkawi, M., & Abbady, A. Q. (2014). Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Molecular biology reports, 41(10), 6887–6898. https://doi.org/10.1007/s11033-014-3575-x

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None